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full length mouse kdm4d sequence  (Addgene inc)


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    Addgene inc full length mouse kdm4d sequence
    Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), <t>Kdm4d-overexpressed</t> (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.
    Full Length Mouse Kdm4d Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+mouse+kdm4d+sequence/pmc12910570-59-6-11?v=Addgene+inc
    Average 93 stars, based on 6 article reviews
    full length mouse kdm4d sequence - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "mTORC1-dependent suppression of autophagic activity in somatic cell nuclear transfer mouse embryos"

    Article Title: mTORC1-dependent suppression of autophagic activity in somatic cell nuclear transfer mouse embryos

    Journal: Reproduction (Cambridge, England)

    doi: 10.1530/REP-25-0338

    Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.
    Figure Legend Snippet: Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.

    Techniques Used: Activity Assay, Activation Assay, Two Tailed Test, Gene Expression



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    full length mouse kdm4d sequence  (Addgene inc)
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    Addgene inc full length mouse kdm4d sequence
    Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), <t>Kdm4d-overexpressed</t> (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.
    Full Length Mouse Kdm4d Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/full+length+mouse+kdm4d+sequence/pmc12910570-59-6-11?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    full length mouse kdm4d sequence - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.

    Journal: Reproduction (Cambridge, England)

    Article Title: mTORC1-dependent suppression of autophagic activity in somatic cell nuclear transfer mouse embryos

    doi: 10.1530/REP-25-0338

    Figure Lengend Snippet: Preimplantation autophagic activity compared in IVF and SCNT embryos. (A) Representative fluorescent images of IVF and SCNT embryos at various times after fertilization or activation. From left to right, 24, 48, 72, and 96 h later were shown, IVF embryos on the upper row, and SCNT embryos on the lower row. GFP-LC3 (green), RFP-LC3ΔG (red). (B) Preimplantation autophagic activity in IVF and SCNT embryos. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), SCNT (orange), lighter-colored areas indicate SEM. Two-tailed t -tests were performed at 36, 48, 60, and 72 h. IVF n = 35, SCNT n = 6. (C) Autophagic activity in IVF and SCNT embryos that were treated to enhance gene expression from 24 to 72 h after insemination or activation. The x -axis shows the elapsed time, and the y -axis shows the relative RFP/GFP values for each embryo based on the 24-h time point. IVF (blue), Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. IVF: n = 12, Kdm4d: n = 38, TSA: n = 18. (D) Autophagic activity up to 48 h after activation in SCNT embryos treated to enhance gene expression. Kdm4d-overexpressed (red), TSA-treated (green), lighter-colored areas indicate SEM. The broken lines indicate embryos that were subjected to the respective treatments, but embryonic development was arrested after the 4-cell stage. Two-tailed t -tests were performed at 48 h for each treatment group. Kdm4d arrest n = 22, TSA arrest n = 35.

    Article Snippet: Briefly, the pcDNA3.1 plasmid carrying the full-length mouse Kdm4d sequence (61553, Addgene, USA) was linearized by XbaI, and it was used as a template for in vitro transcription using mMESSAGE mMACHINE T7 ULTRA Transcription kits (AM1345, Thermo Fisher Scientific).

    Techniques: Activity Assay, Activation Assay, Two Tailed Test, Gene Expression